{"id":198,"date":"2017-12-13T15:23:36","date_gmt":"2017-12-13T07:23:36","guid":{"rendered":"http:\/\/www.wuchangsong.com\/?p=198"},"modified":"2017-12-14T02:36:57","modified_gmt":"2017-12-13T18:36:57","slug":"wgcna%e5%8e%9f%e7%90%86%e5%8f%8a%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"http:\/\/www.wuchangsong.com\/?p=198","title":{"rendered":"WGCNA\u539f\u7406\u53ca\u5e94\u7528"},"content":{"rendered":"<h4>WGCNA\u4ecb\u7ecd\uff1a<\/h4>\n<p>WGCNA\uff08weighted gene co-expression network 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\/>\n\u7b54\uff1a\u53ea\u8981\u6837\u672c\u95f4\u6709\u76f8\u4f3c\u7684\u751f\u7269\u5b66\u610f\u4e49\uff0c\u662f\u53ef\u4ee5\u5408\u5e76\u5728\u4e00\u8d77\u505a\u5206\u6790\u7684\u3002\u4f46\u8981\u6ce8\u610f\uff0c\u4e0d\u540c\u6279\u6b21\u4e4b\u95f4\u7684\u6837\u672c\u662f\u6709\u6279\u6b21\u6548\u5e94\u7684\uff0c\u6240\u4ee5\u53ef\u80fd\u4f1a\u5e26\u6765\u4e00\u4e9b\u8bef\u5dee\uff0c\u4f46\u662f\u662f\u53ef\u4ee5\u653e\u5728\u4e00\u8d77\u5206\u6790\u7684\u3002<\/p>\n<p>\u95ee4\u3001\u76f8\u540c\u6750\u6599\u4e0d\u540c\u5904\u7406\u4e4b\u95f4\uff0c\u53ef\u4ee5\u653e\u5728\u4e00\u8d77\u505aWGCNA\u5206\u6790\u5417\uff1f\u6bd4\u5982\u91cd\u91d1\u5c5e\u548c\u76d0\u78b1\u5904\u7406\u3002<br \/>\n\u7b54\uff1a\u53ef\u4ee5\u7684\u3002\u8fd9\u4e5f\u6b63\u5f0fWGCNA\u5f3a\u5927\u7684\u5730\u65b9\uff0c\u5176\u53ef\u4ee5\u5c06\u4e0d\u540c\u5904\u7406\u7684\u6837\u672c\uff0c\u5408\u5e76\u5728\u4e00\u8d77\u505a\u5206\u6790\u3002\u5176\u4ed6\u65b9\u6cd5\u5219\u4e0d\u4e00\u5b9a\u6709\u8fd9\u4e48\u5f3a\u5927\u7684\u80fd\u529b\uff0c\u6bd4\u5982\u505a\u57fa\u56e0\u8868\u8fbe\u8d8b\u52bf\u5206\u6790\u65f6\uff0c\u5982\u679c\u6837\u672c\u6d89\u53ca\u5230\u591a\u4e2a\u5904\u7406\u4e0d\u540c\u65f6\u671f\u7684\u65f6\u5019\uff0c\u5c31\u4e0d\u597d\u5408\u5e76\u5206\u6790\uff08\u6216\u5408\u5e76\u540e\u96be\u4ee5\u89e3\u8bfb\uff09\u3002\u4f46WGCNA\u7684\u65b9\u6cd5\u5173\u6ce8\u7684\u662f\u8c03\u63a7\u5173\u7cfb\uff0c\u6240\u4ee5\u4e0d\u7ba1\u662f\u591a\u5c11\u4e2a\u5904\u7406\u7ec4\uff0c\u90fd\u53ef\u4ee5\u5f88\u597d\u7684\u6574\u5408\u5728\u4e00\u8d77\u505a\u5206\u6790\u3002<\/p>\n<p>\u95ee5\u3001\u4e0d\u540c\u6279\u6b21\u7684\u6570\u636e\u80fd\u653e\u4e00\u8d77\u505aWGCNA\u5417\uff1f<br \/>\n\u7b54\uff1a\u53ef\u4ee5\u7684\u3002\u867d\u7136\u6709\u6279\u6b21\u7684\u5e72\u6270\uff0c\u4f46\u662f\u5e72\u6270\u5bf9WGCNA\u7f51\u7edc\u6ca1\u6709\u592a\u5927\u5f71\u54cd\u3002\u56e0\u4e3aWGCNA\u4e0d\u662f\u505a\u5dee\u5f02\u5206\u6790\uff0c\u800c\u662f\u57fa\u56e0\u7684\u5171\u8868\u8fbe\u3002\u56e0\u4e3a\u6279\u6b21\u6548\u5e94\u7406\u8bba\u4e0a\u4e0d\u5f71\u54cd\u76f8\u5173\u6027\u3002<\/p>\n<p>\u95ee6\u3001\u4e0d\u540c\u7c7b\u578b\u7684\u6750\u6599\uff0c\u6bd4\u5982\u4eb2\u672c\u548cF1\uff0c\u9002\u5408\u653e\u4e00\u8d77\u8fdb\u884cWGCNA\u4e48\uff1f<br \/>\n\u7b54\uff1a\u5982\u679c\u662f\u4e00\u4e2a\u4f5c\u56fe\u7fa4\u4f53\uff0c\u5f53\u7136\u4eb2\u672c\u4e0eF1\u662f\u53ef\u4ee5\u653e\u5728\u4e00\u8d77\u5206\u6790\u7684\uff0c\u56e0\u4e3a\u4f60\u53ea\u5173\u5fc3\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\uff0c\u6240\u4ee5\u628a\u4eb2\u672c\u52a0\u8fdb\u6765\u662f\u6ca1\u6709\u95ee\u9898\u7684\u3002<\/p>\n<p>\u95ee7\u3001\u6ca1\u6709\u751f\u7269\u5b66\u91cd\u590d\uff0c\u51713\u7ec4\uff0c\u6bcf\u7ec45\u4e2a\u65f6\u95f4\u70b9\u80fd\u591f\u505a\u5417\uff1f<br 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\/>\n\u7b54\uff1a15\u4e2a\u6837\u672c\u8fd9\u4e2a\u662f\u5305\u542b\u4e86\u751f\u7269\u5b66\u91cd\u590d\uff0c\u6bd4\u59825\u4e2a\u65f6\u95f4\u70b93\u4e2a\u91cd\u590d\uff1b\u5728RNA-seq\u91cc\u9762\u5efa\u8bae\u4e0d\u8981\u7528\u4e0d\u540c\u500d\u6027\u6750\u6599\u52a0\u8fdb\u6765\u3002\u9664\u975e\u662f\u6709\u53c2\u8003\u7684\u591a\u500d\u4f53\uff0c\u5982\u679c\u662f\u65e0\u53c2\u7684\u591a\u500d\u4f53\uff0c\u4e0d\u540c\u500d\u6027\u4e4b\u95f4\u5dee\u5f02\u592a\u5927\uff0c\u4f1a\u8ba9\u8c03\u63a7\u7f51\u7edc\u4e0d\u51c6\u786e\u3002\u6240\u4ee5\u7528\u5355\u4e00\u500d\u6027\u7684\u6750\u6599\u505a\u8c03\u63a7\u7f51\u7edc\u4f1a\u66f4\u52a0\u51c6\u786e\u3002<\/p>\n<p>\u95ee9\u3001\u53ef\u4ee5\u5c06RNA-seq\u6570\u636e\u4e0e\u86cb\u767d\u7ec4\u6570\u636e\uff0c\u7532\u57fa\u5316\u6570\u636e\u653e\u4e00\u8d77\u505aWGCNA\u5206\u6790\uff1f<br 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\/>\n\u7b54\uff1a\u8fd9\u4e2a\u662f\u5177\u4f53\u95ee\u9898\u5177\u4f53\u5206\u6790\u3002\u5982\u679c\u4f7f\u7528\u6240\u6709\u7684\u57fa\u56e0\u5206\u6790\uff0c\u4f1a\u5bfc\u81f4\u8fd0\u7b97\u91cf\u975e\u5e38\u5927\u3002\u800c\u4e5f\u4e0d\u662f\u6240\u6709\u7684\u57fa\u56e0\u5728\u8fd9\u4e2a\u5b9e\u9a8c\u4e2d\u90fd\u6709\u751f\u7269\u5b66\u610f\u4e49\uff0c\u6240\u4ee5\u6211\u4eec\u4f1a\u63d0\u524d\u505a\u4e00\u4e9b\u8fc7\u6ee4\u3002<br \/>\n\u4f46\u7528\u4e8e\u5206\u6790\u7684\u57fa\u56e0\u4e0d\u4e00\u5b9a\u662f\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff0c\u6709\u65f6\u53ef\u4ee5\u7528\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u505a\u4e00\u4e2a\u5e76\u96c6\uff0c\u6216\u901a\u8fc7\u8ba1\u7b97\u53d8\u5f02\u7cfb\u6570\u5c06\u53d8\u5f02\u7cfb\u6570\u4f4e\u7684\u57fa\u56e0\u4ee5\u53ca\u4f4e\u8868\u8fbe\u7684\u57fa\u56e0\u53bb\u9664\u3002\u4f46\u6ce8\u610f\uff0c\u5982\u679c\u4f60\u6709\u5173\u5fc3\u7684\u7279\u5b9a\u76ee\u6807\u57fa\u56e0\u7684\u8bdd\uff0c\u5e94\u8be5\u5c3d\u91cf\u7ed9\u4e88\u4fdd\u7559\u3002<\/p>\n<p>\u95ee17\u3001\u5173\u6ce8\u67d0\u4e00\u4e2apathway\u4e0a\u7684\u57fa\u56e0\u4ee5\u53ca\u8c03\u63a7\u56e0\u5b50\u4e4b\u95f4\u7684\u76f8\u5173\u6027\uff0c\u6784\u5efaWGCNA\u7f51\u7edc\u7684\u65f6\u5019\u5c5e\u4e8e\u8fd9\u4e2apathway\u7684\u57fa\u56e0\u6570\u91cf\u592a\u5c11\u4f1a\u4e0d\u4f1a\u5f71\u54cd\u7ed3\u679c\u5462\uff1f<br 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\/>\n\u7b54\uff1a\u7528TOM\u503c\u6765\u6784\u5efa\u77e9\u9635\uff0cTOM\u503c\u5c31\u662f\u4e24\u4e2a\u6837\u672c\u7684\u76f8\u4f3c\u5ea6\uff0c1-TOM\u503c\u5c31\u662f\u4e24\u4e2a\u6837\u672c\u7684\u5dee\u5f02\u5ea6\uff0c\u76f8\u4f3c\u5ea6\u4e0e\u5dee\u5f02\u5ea6\u53ef\u4ee5\u7406\u89e3\u4e3a\u4e00\u4e2a\u4e1c\u897f\uff0c\u5e76\u4e0d\u77db\u76fe\u3002<\/p>\n<p>\u95ee37\u3001\u6a21\u5757\u7279\u5f81\u503c\u548c\u6837\u672c\u6027\u72b6\u76f8\u5173\u5206\u6790\u7684\u5177\u4f53\u65b9\u6cd5\u662f\uff1f<br \/>\n\u7b54\uff1aR\u5305\u7528\u7684\u662f\u8ba1\u7b97\u76f8\u5173\u7cfb\u6570\u7684\u65b9\u6cd5\u3002<\/p>\n<p>\u95ee38\u3001WGCNA\u91cc\u9762\u4e00\u822c\u4f1a\u63d0\u5230hubgene\uff0c\u5982\u4f55\u786e\u5b9ahubgene?<br \/>\n\u7b54\uff1a\u5728WGCNA\u5206\u6790\u91cc\u9762\uff0c\u6bcf\u4e2a\u57fa\u56e0\u90fd\u4f1a\u8ba1\u7b97\u8fde\u901a\u6027\uff0c\u8fde\u901a\u6027\u9ad8\u7684\u5c31\u662fhubgene\u3002<\/p>\n<p>\u95ee39\u3001\u5728R\u4e2d\u5b89\u88c5\u201c\u201dWGCNA\u201c\u201d\u8bf4\u4e0d\u9002\u5408R3.3.1\uff0c\u90a3\u9002\u5408\u54ea\u4e2a\u7248\u672c\uff1f<br \/>\n\u7b54\uff1aWGCNA\u5e94\u8be5\u662f\u6240\u6709\u7248\u672c\u90fd\u9002\u5408\uff0c\u5982\u679c\u7248\u672c\u6ca1\u6709\u53ef\u4ee5\u8003\u8651\u964d\u4f4eR\u8f6f\u4ef6\u7684\u7248\u672c\uff0c\u8fd9\u4e2a\u5bf9\u5206\u6790\u6ca1\u6709\u5f71\u54cd\u3002\u56e0\u4e3a\u4e0d\u540cR\u7248\u672c\u662f\u4e00\u6837\u7684\u3002<\/p>\n<p>\u95ee40\u3001\u7528STEM\u5206\u6790\u7684\u65f6\u5019\u62df\u5408\u591a\u5c11\u4e2a\u6a21\u578b\u5408\u9002\uff1f<br \/>\n\u7b54\uff1a\u5efa\u8bae\u4e0d\u8981\u8d85\u8fc720\u4e2a\u3002\u6a21\u5757\u592a\u591a\u4e0d\u597d\u5206\u6790\u3002<\/p>\n<p>\u53c2\u8003\u7f51\u7ad9\uff1a<\/p>\n<p><a href=\"http:\/\/tiramisutes.github.io\/2016\/09\/14\/WGCNA.html\">http:\/\/tiramisutes.github.io\/2016\/09\/14\/WGCNA.html<\/a><\/p>\n<p><a href=\"http:\/\/www.jianshu.com\/p\/94b11358b3f3\">http:\/\/www.jianshu.com\/p\/94b11358b3f3<\/a><\/p>\n<p><a href=\"http:\/\/www.omicshare.com\/class\/home\/index\/classdetail?id=20\">http:\/\/www.omicshare.com\/class\/home\/index\/classdetail?id=20<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>WGCNA\u4ecb\u7ecd\uff1a WGCNA\uff08weighted gene co-expression network analysis\uff0c\u6743\u91cd\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\u5206\u6790\uff09\u662f\u4e00\u79cd\u5206\u6790\u591a\u4e2a\u6837\u672c\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u5c06\u8868\u8fbe\u6a21\u5f0f\u76f8\u4f3c\u7684\u57fa\u56e0\u8fdb\u884c\u805a\u7c7b\uff0c\u5e76\u5206\u6790\u6a21\u5757\u4e0e\u7279\u5b9a\u6027\u72b6\u6216\u8868\u578b\u4e4b\u95f4\u7684\u5173\u8054\u5173\u7cfb\uff0c\u56e0\u6b64\u5728\u75be\u75c5\u4ee5\u53ca\u5176\u4ed6\u6027\u72b6\u4e0e\u57fa\u56e0\u5173\u8054\u5206\u6790\u7b49\u65b9\u9762\u7684\u7814\u7a76\u4e2d\u88ab\u5e7f\u6cdb\u5e94\u7528\u3002 WGCNA\u7b97\u6cd5\u662f\u6784\u5efa\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\u7684\u5e38\u7528\u7b97\u6cd5\uff08\u8be6\u89e3\uff1ahttp:\/\/www.jianshu.com\/p\/94b11358b3f3\uff09\u3002WGCNA\u7b97\u6cd5\u9996\u5148\u5047\u5b9a\u57fa\u56e0\u7f51\u7edc\u670d\u4ece\u65e0\u5c3a\u5ea6\u5206\u5e03\uff0c\u5e76\u5b9a\u4e49\u57fa\u56e0\u5171\u8868\u8fbe\u76f8\u5173\u77e9\u9635\u3001\u57fa\u56e0\u7f51\u7edc\u5f62\u6210\u7684\u90bb\u63a5\u51fd\u6570\uff0c\u7136\u540e\u8ba1\u7b97\u4e0d\u540c\u8282\u70b9\u7684\u76f8\u5f02\u7cfb\u6570\uff0c\u5e76\u636e\u6b64\u6784\u5efa\u5206\u5c42\u805a\u7c7b\u6811(hierarchical clustering tree)\uff0c\u8be5\u805a\u7c7b\u6811\u7684\u4e0d\u540c\u5206\u652f\u4ee3\u8868\u4e0d\u540c\u7684\u57fa\u56e0\u6a21\u5757(module)\uff0c\u6a21\u5757\u5185\u57fa\u56e0\u5171\u8868\u8fbe\u7a0b\u5ea6\u9ad8\uff0c\u800c\u5206\u5c5e\u4e0d\u540c\u6a21\u5757\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u7a0b\u5ea6\u4f4e\u3002\u6700\u540e\uff0c\u63a2\u7d22\u6a21\u5757\u4e0e\u7279\u5b9a\u8868\u578b\u6216\u75be\u75c5\u7684\u5173\u8054\u5173\u7cfb\uff0c\u6700\u7ec8\u8fbe\u5230\u9274\u5b9a\u75be\u75c5\u6cbb\u7597\u7684\u9776\u70b9\u57fa\u56e0\u3001\u57fa\u56e0\u7f51\u7edc\u7684\u76ee\u7684\u3002\u5728\u8be5\u65b9\u6cd5\u4e2dmodule\u88ab\u5b9a\u4e49\u4e3a\u4e00\u7ec4\u5177\u6709\u7c7b\u4f3c\u8868\u8fbe\u8c31\u7684\u57fa\u56e0\uff0c\u5982\u679c\u67d0\u4e9b\u57fa\u56e0\u5728\u4e00\u4e2a\u751f\u7406\u8fc7\u7a0b\u6216\u4e0d\u540c\u7ec4\u7ec7\u4e2d\u603b\u662f\u5177\u6709\u76f8\u7c7b\u4f3c\u7684\u8868\u8fbe\u53d8\u5316\uff0c\u90a3\u4e48\u6211\u4eec\u6709\u7406\u7531\u8ba4\u4e3a\u8fd9\u4e9b\u57fa\u56e0\u5728\u529f\u80fd\u4e0a\u662f\u76f8\u5173\u7684\uff0c\u53ef\u4ee5\u628a\u4ed6\u4eec\u5b9a\u4e49\u4e3a\u4e00\u4e2a\u6a21\u5757(module)\u3002\u8fd9\u4f3c\u4e4e\u6709\u70b9\u7c7b\u4f3c\u4e8e\u8fdb\u884c\u805a\u7c7b\u5206\u6790\u6240\u5f97\u5230\u7ed3\u679c\uff0c\u4f46\u4e0d\u540c\u7684\u662f\uff0cWGCNA\u7684\u805a\u7c7b\u51c6\u5219\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\uff0c\u800c\u975e\u5e38\u89c4\u7684\u805a\u7c7b\u65b9\u6cd5(\u5982\u5229\u7528\u6570\u636e\u95f4\u7684\u51e0\u4f55\u8ddd\u79bb)\uff0c\u56e0\u6b64\u8be5\u65b9\u6cd5\u6240\u5f97\u51fa\u7684\u7ed3\u679c\u5177\u6709\u66f4\u9ad8\u7684\u53ef\u4fe1\u5ea6\u3002\u5f53\u57fa\u56e0module\u88ab\u5b9a\u4e49\u51fa\u6765\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u5229\u7528\u8fd9\u4e9b\u7ed3\u679c\u505a\u5f88\u591a\u8fdb\u4e00\u6b65\u7684\u5de5\u4f5c\uff0c\u5982\u5173\u8054\u6027\u72b6\uff0c\u4ee3\u8c22\u901a\u8def\u5efa\u6a21\uff0c\u5efa\u7acb\u57fa\u56e0\u4e92\u4f5c\u7f51\u7edc\u7b49\u3002 &nbsp; WGCNA\u7684\u7528\u5904\uff1a &nbsp; \u8fd9\u7c7b\u5904\u4e8e\u8c03\u63a7\u7f51\u7edc\u4e2d\u5fc3\u7684\u57fa\u56e0\u79f0\u4e3a\u6838\u5fc3\u57fa\u56e0\uff08hub gene\uff09\uff0c\u8fd9\u7c7b\u57fa\u56e0\u901a\u5e38\u662f\u8f6c\u5f55\u56e0\u5b50\u7b49\u5173\u952e\u7684\u8c03\u63a7\u56e0\u5b50\uff0c\u662f\u503c\u5f97\u6211\u4eec\u4f18\u5148\u6df1\u5165\u5206\u6790\u548c\u6316\u6398\u7684\u5bf9\u8c61\u3002 \u5728\u7f51\u7edc\u4e2d\uff0c\u88ab\u8c03\u63a7\u7ebf\u8fde\u63a5\u7684\u57fa\u56e0\uff0c\u5176\u8868\u8fbe\u6a21\u5f0f\u662f\u76f8\u4f3c\u7684\u3002\u90a3\u4e48\u5b83\u4eec\u6f5c\u5728\u6709\u76f8\u4f3c\u7684\u529f\u80fd\u3002\u6240\u4ee5\uff0c\u5728\u8fd9\u4e2a\u7f51\u7edc\u4e2d\uff0c\u5982\u679c\u7ebf\u6761\u4e00\u7aef\u7684\u57fa\u56e0\u529f\u80fd\u662f\u5df2\u77e5\u7684\uff0c\u90a3\u4e48\u5c31\u53ef\u4ee5\u9884\u6d4b\u7ebf\u6761\u53e6\u4e00\u7aef\u7684\u529f\u80fd\u672a\u77e5\u7684\u57fa\u56e0\u4e5f\u6709\u76f8\u4f3c\u7684\u529f\u80fd\u3002 &nbsp; 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