{"id":460,"date":"2019-10-25T18:09:36","date_gmt":"2019-10-25T10:09:36","guid":{"rendered":"http:\/\/www.wuchangsong.com\/?p=460"},"modified":"2019-10-25T18:09:36","modified_gmt":"2019-10-25T10:09:36","slug":"prodigal%e6%b3%a8%e9%87%8a%e5%8e%9f%e6%a0%b8%e5%9f%ba%e5%9b%a0%e7%bb%84","status":"publish","type":"post","link":"http:\/\/www.wuchangsong.com\/?p=460","title":{"rendered":"Prodigal\u6ce8\u91ca\u539f\u6838\u57fa\u56e0\u7ec4"},"content":{"rendered":"<p>\u4e0b\u8f7d\u5730\u5740\uff1ahttps:\/\/github.com\/hyattpd\/prodigal\/releases\/<br \/>\n\u4e0b\u8f7d\u6e90\u7801\u5305\uff1aProdigal-2.6.3.tar.gz<\/p>\n<pre> tar -zxvf Prodigal-2.6.3.tar.gz\r\ncd Prodigal-2.6.3\r\nmake install\r\n#\u6dfb\u52a0\u73af\u5883\u53d8\u91cf\r\nprodigal -a UBA705.pep -d UBA705.cds -f gff -g 11  -o UBA705.gff -p single -s UBA705.stat -i UBA705.fasta &gt; prodigal.log<\/pre>\n<p>-a \u662f\u8f93\u51fa\u6c28\u57fa\u9178\u6587\u4ef6<\/p>\n<p>-c \u4e0d\u5141\u8bb8\u57fa\u56e0\u4e00\u8fb9\u65ad\u5f00\uff0c\u4e5f\u5c31\u662f\u8981\u6c42\u5b8c\u6574\u7684orf\uff0c\u6709\u8d77\u59cb\u548c\u7ec8\u6b62\u7ed3\u6784<\/p>\n<p>-d \u8f93\u51fa\u9884\u6d4b\u57fa\u56e0\u7684\u5e8f\u5217\u6587\u4ef6<\/p>\n<p>-f \u9009\u62e9\u8f93\u51fa\u6587\u4ef6\u683c\u5f0f\uff0c\u6709gbk,gff,\u548csco\u683c\u5f0f\u53ef\u4f9b\u9009\u62e9<\/p>\n<p>-g \u6307\u5b9a\u5bc6\u7801\u5b50\uff0c\u539f\u6838\u4e3a\u7b2c11\u5957<\/p>\n<p>-i \u8f93\u5165\u6587\u4ef6\uff0c\u5373\u9700\u8981\u9884\u6d4b\u7684\u57fa\u56e0\u7ec4\u5e8f\u5217\u6587\u4ef6<\/p>\n<p>-m \u5c4f\u853d\u57fa\u56e0\u7ec4\u4e2d\u7684N\u78b1\u57fa<\/p>\n<p>-o \u8f93\u51fa\u6587\u4ef6\uff0c\u9ed8\u8ba4\u4e3a\u5c4f\u5e55\u8f93\u51fa<\/p>\n<p>-p \u9009\u62e9\u65b9\u5f0f\uff0c\u662f\u5355\u83cc\u8fd8\u662fmeta\u6837\u54c1<\/p>\n<p>-q \u4e0d\u8f93\u51fa\u9519\u8bef\u4fe1\u606f\u5230\u5c4f\u5e55<\/p>\n<p>-t \u6307\u5b9a\u8bad\u7ec3\u96c6<\/p>\n<p>-s \u8f93\u51fa\u6240\u6709\u6f5c\u5728\u57fa\u56e0\u4ee5\u53ca\u5206\u503c\u5230\u4e00\u4e2a\u6587\u4ef6\u4e2d<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e0b\u8f7d\u5730\u5740\uff1ahttps:\/\/github.com\/hyattpd\/prodigal\/releases\/ \u4e0b\u8f7d\u6e90\u7801\u5305\uff1aProdigal-2.6.3.tar.gz tar -zxvf Prodigal-2.6.3.tar.gz cd Prodigal-2.6.3 make install #\u6dfb\u52a0\u73af\u5883\u53d8\u91cf prodigal -a UBA705.pep -d UBA705.cds -f gff -g 11 -o UBA705.gff -p single -s UBA705.stat -i UBA705.fasta &gt; prodigal.log -a \u662f\u8f93\u51fa\u6c28\u57fa\u9178\u6587\u4ef6 -c \u4e0d\u5141\u8bb8\u57fa\u56e0\u4e00\u8fb9\u65ad\u5f00\uff0c\u4e5f\u5c31\u662f\u8981\u6c42\u5b8c\u6574\u7684orf\uff0c\u6709\u8d77\u59cb\u548c\u7ec8\u6b62\u7ed3\u6784 -d \u8f93\u51fa\u9884\u6d4b\u57fa\u56e0\u7684\u5e8f\u5217\u6587\u4ef6 -f \u9009\u62e9\u8f93\u51fa\u6587\u4ef6\u683c\u5f0f\uff0c\u6709gbk,gff,\u548csco\u683c\u5f0f\u53ef\u4f9b\u9009\u62e9 -g \u6307\u5b9a\u5bc6\u7801\u5b50\uff0c\u539f\u6838\u4e3a\u7b2c11\u5957 -i \u8f93\u5165\u6587\u4ef6\uff0c\u5373\u9700\u8981\u9884\u6d4b\u7684\u57fa\u56e0\u7ec4\u5e8f\u5217\u6587\u4ef6 -m \u5c4f\u853d\u57fa\u56e0\u7ec4\u4e2d\u7684N\u78b1\u57fa -o \u8f93\u51fa\u6587\u4ef6\uff0c\u9ed8\u8ba4\u4e3a\u5c4f\u5e55\u8f93\u51fa -p \u9009\u62e9\u65b9\u5f0f\uff0c\u662f\u5355\u83cc\u8fd8\u662fmeta\u6837\u54c1 -q \u4e0d\u8f93\u51fa\u9519\u8bef\u4fe1\u606f\u5230\u5c4f\u5e55 -t \u6307\u5b9a\u8bad\u7ec3\u96c6 -s \u8f93\u51fa\u6240\u6709\u6f5c\u5728\u57fa\u56e0\u4ee5\u53ca\u5206\u503c\u5230\u4e00\u4e2a\u6587\u4ef6\u4e2d<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"_links":{"self":[{"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=\/wp\/v2\/posts\/460"}],"collection":[{"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=460"}],"version-history":[{"count":1,"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=\/wp\/v2\/posts\/460\/revisions"}],"predecessor-version":[{"id":461,"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=\/wp\/v2\/posts\/460\/revisions\/461"}],"wp:attachment":[{"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=460"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=460"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.wuchangsong.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=460"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}