MLST细菌分型

多位点序列分型(multilocus sequence typing,MLST)是一种基于核酸序列测定的细菌分型方法。这种方法通过PCR扩增多个管家基因内部片段并测定其序列,分析菌株的变异。

依赖conda安装

conda install -c conda-forge -c bioconda -c defaults mlst
mlst contigs.fa
contigs.fa  neisseria  11149  abcZ(672) adk(3) aroE(4) fumC(3) gdh(8) pdhC(4) pgm(6)

mlst genomes/*
genomes/6008.fna        saureus         239  arcc(2)   aroe(3)   glpf(1)   gmk_(1)   pta_(4)   tpi_(4)   yqil(3)
genomes/strep.fasta.gz  ssuis             1  aroA(1)   cpn60(1)  dpr(1)    gki(1)    mutS(1)   recA(1)   thrA(1)
genomes/NC_002973.gbk   lmonocytogenes    1  abcZ(3)   bglA(1)   cat(1)    dapE(1)   dat(3)    ldh(1)    lhkA(3)
genomes/L550.gbk.bz2    leptospira      152  glmU(26)  pntA(30)  sucA(28)  tpiA(35)  pfkB(39)  mreA(29)  caiB(29)

mlst --scheme neisseria NM*
NM003.fa   neisseria  4821  abcZ(222)  adk(3)  aroE(58)  fumC(275)  gdh(30)  pdhC(5)  pgm(255)
NM005.gbk  neisseria  177   abcZ(7)    adk(8)  aroE(10)  fumC(38)   gdh(10)  pdhC(1)  pgm(20)
NM011.fa   neisseria  11    abcZ(2)    adk(3)  aroE(4)   fumC(3)    gdh(8)   pdhC(4)  pgm(6)
NMC.gbk.gz neisseria  8     abcZ(2)    adk(3)  aroE(7)   fumC(2)    gdh(8)   pdhC(5)  pgm(2)

mlst --legacy --scheme neisseria *.fa
FILE      SCHEME     ST    abcZ  adk  aroE  fumC  gdh  pdhC  pgm
NM003.fa  neisseria  11    2     3    4     3       8     4    6
NM009.fa  neisseria  11149 672   3    4     3       8     4    6
MN043.fa  neisseria  11    2     3    4     3       8     4    6
NM051.fa  neisseria  11    2     3    4     3       8     4    6
NM099.fa  neisseria  1287  2     3    4    17       8     4    6
NM110.fa  neisseria  11    2     3    4     3       8     4    6

链接:Mlst官网

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